The invention belongs to the field of macromolecule composite conductive materials, and discloses a carbon nanotube-nano-crystalline cellulose-polyvinyl alcohol composite conductive gel and a preparation method and application thereof. The gel is prepared from the following steps: a: preparing carbon nanotube dispersion liquid; b: preparing nano-crystalline cellulose suspension; c: taking the mixture of the carbon nanotube and the nano-crystalline cellulose, adding the mixture into polyvinyl alcohol aqueous solution, adding a crosslinking agent, stirring until gel is formed to obtain the gel. The gel can be used for preparing flexible conductive materials and has a better application prospect.