The invention discloses an MrgprX2 high-expression reconstituted cell and MrgprX2 high-expression membrane receptor stationary phase and a preparation method and application thereof. The reconstituted cell uses HEK293 cells as host cells for transfecting expression carriers containing MrgprX2 full-length genes; the MrgPrX2 receptor macromolecules can be stably expressed on the cell membrane; in addition, the complete receptor biological activity is realized. The MrgprX2 high-expression membrane receptor stationary phase is manufactured by adsorbing cytomembranes of MrgprX2 high-expression reconstituted cells by activated silica gel; the application to the specific screening and identification of MrgprX2 triggered anaphylactoid ingredients can be realized. Through practical application, the condition that the sinomenine hydrochloride can be specifically combined with MrgprX2 is discovered. Meanwhile, the cell level allergic experiments prove that the sinomenine hydrochloride can stimulate mastocytes to release histamine and beta-hexosaminidase; the result proves that the MrgprX2 high-expression membrane receptor stationary phase can really be applied to the specific identification of the anaphylactoid ingredients.