The invention discloses a battery voltage acquisition and calibration circuit. The battery voltage acquisition and calibration circuit comprises a battery voltage acquisition module and further comprises an MCU (Microprogrammed Control Unit), a multi-path standard voltage generation circuit, a constant-current source circuit and a voltage following circuit; the MCU is used for controlling the multi-path standard voltage generation circuit to generate a plurality of paths of standard voltage and battery voltage is simulated through the constant-current source circuit and is acquired through the battery voltage acquisition module; the MCU is connected with the battery voltage acquisition module and is used for transmitting a voltage value of the standard voltage to the battery voltage acquisition module; the battery voltage acquisition module is used for comparing the voltage value of the acquired standard voltage with a standard voltage value transmitted by the MCU, and calculating errors and automatically calibrating, and an output band loading capability is increased through the voltage following circuit; the aim of automatically acquiring and calibrating voltage of a single battery or a plurality of batteries is realized; meanwhile, the battery voltage acquisition and calibration circuit is simple in structure and low in cost and has good economic value. The battery voltage acquisition and calibration circuit disclosed by the invention is widely applied to the technical field of battery management.