The invention discloses an MAC layer queue scheduling method of a TDMA ad hoc network for mainly solving the problems of poor fairness, too long delay and waste of system resources in the prior art. The scheme is as follows: selecting a business time slot of a present node, and performing queue scheduling: 1, judging whether a data frame with a waiting time exceeding a waiting threshold, judging whether a queue with a queue length exceeding a queue length critical value, if both of the two situations do not exist, counting the number of neighbor nodes having the maximum transmission unit value; 2, comparing queue priority and queue length in N neighbor nodes, and selecting a neighbor node to serve as destination neighbor node; and 3, determining the number of the sent data frames according to the maximum transmission unit value of the destination neighbor node, and sending the data frames in a frame aggregation manner. The MAC layer queue scheduling method disclosed by the invention has the advantages of high fairness, low delay, timely data processing and large throughput, and can be used for providing a scheduling mode with delay and throughput guarantee for MAC layer data transmission.