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Invention Publication
CN106811427A 角度双加氧酶基因dpeA1A2及其应用 失效 - 权利终止

角度双加氧酶基因dpeA1A2及其应用
Abstract:
本发明公开了角度双加氧酶基因dpeA1A2及其应用。一种角度双加氧酶基因dpeA1A2，其由SEQ ID NO.1所示的大小为1383bp的角度双加氧酶大亚基编码基因dpeA1、SEQ ID NO.2所示的大小为561bp的角度双加氧酶小亚基编码基因dpeA2以及二者之间的连接序列GATG组成。本发明提供的DpeA1A2BC全酶能在72hr内完全降解100mg/L的二苯醚；此外DpeA1A2BC全酶还能在72hr内完全降解100mg/L的2-苯氧基苯甲酸(2-PBA)。因此角度双加氧酶基因dpeA1A2在构建降解二苯醚或2-苯氧基苯甲酸转基因作物中应用。DpeA1A2BC全酶在降解二苯醚和2-PBA中应用。
Abstract(English):

The invention discloses an angle dioxygenase gene dpeA1A2 and application thereof. The angle dioxygenase gene dpeA1A2 consists of an angle dioxygenase large-subunit coding gene dpeA1 with a size of 1383bp as shown by SEQ ID NO.1 and an angle dioxygenase gene small-subunit coding gene dpeA2 with a size of 561bp as shown by SEQ ID NO.1 and a connection sequence GATG between dpe A1 and dpeA2. DpeA1A2BC holoenzyme provided by the invention can completely degrade 100mg/L of diphenyl ether in 72h; and additionally, the DpeA1A2BC holoenzyme can also completely degrade 100mg/L of 2-PBA in 72h. Therefore, the angle dioxygenase gene dpeA1A2 is used for establishing a transgenic crop for degrading the diphenyl ether and 2-PBA. The DpeA1A2BC holoenzyme is used for degrading the diphenyl ether and the 2-PBA.

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