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Invention Grant
CN106811466B 一种我国小麦根部禾谷多黏菌分型及其鉴定的方法 失效 - 权利终止

一种我国小麦根部禾谷多黏菌分型及其鉴定的方法
Abstract:
本发明涉及一种我国小麦根部禾谷多黏菌分型及其鉴定的方法，所述方法为利用禾谷多黏菌的特异性正向引物PSP2(f)和反相引物ITS4(r)对小麦根部基因组DNA进行PCR扩增，判断小麦根部是否存在禾谷多黏菌。再利用正向引物NS7(f)和反向引物ITS.CN(r)，及正向引物Pg.F2(f)和反向引物Pg.R2(r)确定禾谷多黏菌的分型。
Abstract(English):

The invention relates to a method of typing and identifying polymyxa graminis at roots of wheat in China. The method comprises the following steps of: performing PCR amplification on genomic DNA of the roots of the wheat by using a specific positive primer PSP2(f) and a reverse primer ITS4(r) of polymyxa graminis to judge whether the roots of the wheat have polymyxa graminis or not; and then determining types of the polymyxa graminis by employing a positive primer NS7(f) and a reverse primer ITS.CN(r) as well as a positive primer Pg.F2(f) and a reverse primer Pg.R2(r).

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