The invention belongs to the technical field of biomedicine, and particularly relates to a method for preparing an iPSCs (Induced Pluripotent Stem Cells) genetic neuron capable of being traced in real time. The method comprises the following steps of knocking a reporter gene mRFP (monomeric Red Fluorescent Protein) into a TUBB3 (Tubulin Beta-3) allel by adopting a Crispr/Cas9 gene editing technique, connecting a coding sequence of the TUBB3 with a coding sequence of the mRFP through a 2A peptide chain, and further making and marking human IPSCs containing a TUBB3-2A-mRFP sequence as hiPSCs-TUBB3-mRFP; efficiently differentiating the finally made hiPSCs-TUBB3-mRFP into a neuron. According to the method provided by the invention, through an induced directional differentiation program, the hiPSCs-TUBB3-mRFP is efficiently differentiated into the neuron; thus, the experimental foundation is provided for the high-flux and specific neuron sorting of a flow cytometer; the technical platform is further provided for screening a medicine for a nervous system and detecting the neurogenetic toxicity of a compound/environmental toxin.