The invention provides an antifouling paint and a preparation method and applications thereof. The antifouling paint comprises the following components in parts by weight: 55 to 60 parts of modified polyester resin, 0.3 to 0.5 part of hydroxyl modified siloxane, 0.6 to 0.9 part of titanium dioxide, 6.0 to 8.0 parts of curing agent, 0.2 to 0.3 part of leveling agent, 0.1 to 0.2 part of dispersant, 0.1 to 0.2 part of antifoaming agent, 2.5 to 4.0 parts of pigment, 1.5 to 2.0 parts of epoxy phosphate, 7.0 to 8.0 parts of cyclohexanone, 8.0 to 9.0 parts of propylene glycol methyl ether acetate, 3.0 to 4.0 parts of ethylene glycol monobutyl ether, 4.0 to 5.0 parts of n-butanol, and 5.0 to 7.0 parts of solubilizer. The antifouling paint has good hardness and compactness, thus the antifouling performance of the paint is enhanced, the antifouling effect is especially good for the oily sticky substances, and the paint can be widely applied on the surfaces of household kitchenware, machines in factories, and the like.