The invention discloses a resin embedding method of light transmittance biological tissues. The tissues are embedded with an embedding medium with the refractive index matched with the tissues in order to obtain resin embedded tissues with certain transparency, wherein the embedding medium is a polymer formed through initiator initiated polymerization of polymerization monomers. The resin embedding method of the light transmittance biological tissues guarantees that the resin provides solid support, provides certain light transmittance for the biological tissues, and allows the depth of sample imaging to be increased and the Z-axial cutting thickness to be increased during cutting imaging of the tissues, so the tissue imaging time is greatly shortened.