The invention relates to a preparation method of antibacterial and antistatic alpaca wool and belongs to the technical field of preparation and application of functional alpaca wool. An antibacterial and antistatic finishing liquid is prepared by treating silver-loaded nanometer titanium dioxide powder with a silane coupling agent and then is used for finishing the alpaca wool. The obtained alpaca wool has good antibacterial performance and antistatic performance, has the antibacterial rates to staphylococcus aureus and escherichia coli reaching 95-99 percent and 93-97 percent respectively and has the specific resistance of the fiber surface lowered to 10-15 omega from 3.0*10<4> omega; and in addition, the fracture strength of the alpaca wool has no loss basically.