The invention discloses a stropharia rugoso-annulata stereo cultivation method with biological reactions of straw barreled bars, and belongs to the technical field of stropharia rugoso-annulata cultivation. According to the technical scheme, the technical problem to be solved is to provide a stropharia rugoso-annulata cultivation method which is convenient for industrialized management with improvement of output and quality and mass utilization of crop straws. The technical scheme is implemented by the following steps that the first step is material preparation; the second step is to divide corn straws into a long part and a short part; the third step is that the long corn straws are tiled side by side, then leaf mold, corn cobs, other crop straws, shrub branches and crop straw fermentation fines are laid on the long corn straws in sequence, then stropharia rugoso-annulata culture is arranged, wherein the short corn straws are stacked on both sides of the culture, and then the other crop straws, the corn cobs, the leaf mold, and the long corn straws are laid in sequence; the fourth step is bundling; the fifth step is to tile the barreled bars on the ground for spawn running; the sixth step is to move the barreled bars into a sunshade for triangular stereo fruiting; the seventh step is timely harvesting.