The invention relates to the technical field of recombinant virus genetic engineering, in particular to a multi-epitope vaccine candidate strain capable of preventing human adenovirus type 3-human adenovirus type 55-human adenovirus type 7 at the same time. Human adenovirus type 55 neutralization antigenic epitope 55R2 and human adenovirus type 7 neutralization antigenic epitope 7R5 are inserted into an HVR2 area and an HVR5 area of a human adenovirus type 3 hexon correspondingly. The vaccine candidate strain can induce immunoreaction of resisting the human adenovirus type 3, the human adenovirus type 7 and the human adenovirus type 55 at the same time. The vaccine candidate strain can be used for preparing trivalent vaccine for preventing the human adenovirus type 3, the human adenovirus type 7 and the human adenovirus type 55.