The invention relates to a genetic ant colony algorithm based determination method for a water cool storage air-conditioning system energy-saving optimal control strategy. An energy consumption model of a water cool storage air-conditioning system is built and serves as an objective function, the advantages of high convergence rate and high accuracy after combination of a genetic algorithm and an ant colony algorithm are used for conducting optimizing, a globally optimal solution is found and serves as an optimum control parameter, and the energy-saving optimal control strategy is determined.