The invention discloses a full-automatic puncture needle positioner. The positioner comprises a puncture needle locating frame and a locating frame adjusting mechanism. The locating frame adjusting mechanism comprises, in a vertically successive manner, a feeding and discharge mechanism, an angle adjusting mechanism, a radian adjusting mechanism and a horizontal position adjusting mechanism, which are installed on an installation rack. When installation rack rises, the feeding and discharge mechanism, the angle adjusting mechanism, the radian adjusting mechanism and the horizontal position adjusting mechanism can orderly perform feeding, angle adjustment, radian adjustment and horizontal position adjustment of puncture needles on the puncture needle locating frame. Therefore, according to an image of CT scanning, the locating frame adjusting mechanism can accurately adjust the puncture needle locating frame and enable the puncture needles to arrive at accurate positions, thereby simplifying work of medical staff and achieving higher accuracy. Medical operators can directly perform puncturing according to the positions of the puncture needles.