The invention discloses an organosilicone-modified polyurethane anti-pilling finishing agent, a preparation method and applications. The preparation method comprises the following steps: by taking octamethylcyclotetrasiloxane and 1,3-bis(3-aminopropyl)-1,1,3,3-tetramethyldisiloxane as raw materials, preparing double amino-terminated silicon oil under the catalysis action of tetramethyl ammonium hydroxide; by taking isophorone diisocyanate, dehydrated polyethylene glycol, diethylene glycol and N-methyldiethanolamine as raw materials, preparing a polyurethane prepolymer under the catalysis action of dibutyltin dilaurate; and leading the prepared double amino-terminated silicon oil to generate copolymerization reaction with the polyurethane prepolymer, so as to prepare the organosilicone-modified polyurethane anti-pilling finishing agent. The product can be crosslinked into a film on the surfaces of fibers, can limit the slippage of fibers in textiles, can lead ends of fibers to be adhered onto the surface of the textiles to reduce pilling, and is difficult in pilling in use; in addition, the influence of resin finishing on the wearability of the textiles can also be improved.