The invention belongs to the technical field of biological functional carboxylic acid complexes, and in particular relates to an oxadiazole carboxylic acid metal complex as well as a preparation method and application thereof. The structural formula of the metal complex is described in the description, wherein when M is Ag, R1=COOH, R2 does not exist, and R3=H2O; when M is Cu, R1=COO<->, R2=H2O, and R3 does not exist. The oxadiazole carboxylic acid metal complex has the characteristics of being simple to synthesize, convenient to purify, higher in yield and good in stability in the air; the preparation steps are simple and easy to implement; the oxadiazole carboxylic acid metal complex can be used for preparing a urease inhibitor, and has very high urease inhibiting activity; the inhibiting ability of the oxadiazole carboxylic acid metal complex is superior to a positive control drug.