Provided are a hemostatic material, a preparation method thereof and a use thereof. The injectable type self-healing hemostatic material is obtained by self-assembling macromolecular microgel particles with surface charges through electrostatic interaction between the particles or the particles and macromolecules. The electrostatic action is physical crosslinking and has reversibility, and no micromolecular crosslinking agent is introduced during the injecting and curing process, so the obtained hemostatic material has good mechanical strength, and can achieve rapid hemostasis for bleeding tissue with large bleeding volume and high blood pressure.