The invention provides a method for improving and acquiring the original mura accuracy of an LCD panel. The method comprises steps of step 10, controlling the LCD panel to display the low gray scale, shooting an original mura image of the LCD panel under the first backlight brightness and calculating a corresponding gray scale compensation value based on the brightness of the central region of the LCD panel under the first backlight brightness; step 20, displaying a middle gray scale, shooting an original mura image of the LCD panel under the second backlight brightness and calculating a corresponding gray scale compensation value; and step 30, displaying a high gray scale, shooting an original mura image of the LCD panel under the third backlight brightness and calculating a corresponding gray scale compensation value, wherein the brightness of the central region of the LCD panel is the same under the first, second and third backlight brightness. According to the invention, the mura of each gray scale can be clearly displayed under the corresponding backlight brightness, thereby facilitating calculation of precise compensation data.