The invention provides an LVDS (Low-Voltage Differential Signaling) output protection and prevention hot plugging circuit used for accessing an LVDS signal to a display screen. The circuit comprises a data protection circuit, which is configured to transmit an LVDS data signal, and prevent ESD (Electro-Static discharge) and reduce EMI (Electromagnetic Interference) protection for the LVDS data signal; a power selection circuit, which is configured to transmit an LVDS power signal and divide the LVDS power signal into two paths and output the signal to supply power to the supply voltage selected to be matched by the display screen; and an anti-hot plug circuit, which is configured to detect a detection pin inserted into a display screen state, control the power supply of the data protection circuit according to the voltage magnitude of the detection pin and control the power selection circuit to be outputted to the amplitude of the display screen. The LVDS output protection and prevention hot plugging circuit provided by the invention provides the protection of signals and power supplies when a board card which needs to output the LVDS signal outputs the signals, prevents the damage of components and chips caused by hot plug or wire rod anti-plug.