The invention discloses a monoclonal antibody hybridoma cell line GHY1 with alachlor, acetochlor, propachlor and butyraldehyde and application thereof, and belongs to the field of food safety immunodetection. The monoclonal antibody hybridoma cell line GHY1 with the alachlor, acetochlor, propachlor and butyraldehyde has been preserved in a general microorganism center of China Committee for Culture Collection of Microorganisms, referred to as CGMCC, and preservation number is CGMCC No.12030. The monoclonal antibody is secreted and produced by the CGMCC No.12030 monoclonal antibody hybridoma cell line GHY1 with the alachlor, acetochlor, propachlor and butyraldehyde. The monoclonal antibody secreted by the cell line has good specificity and detection sensitivity to alachlor, acetochlor, propachlor and butyraldehyde, and has practical application value, and the detection on alachlor, acetochlor, propachlor and butyraldehyde residues in water, fruit and vegetable and cereal is realized.