The invention discloses an in vitro dynamic culture system which is composed of a plurality of working stations with same structures, wherein each working station comprises an upper loading sample and a combined sample slot; the exterior of the combined sample slot is made of a hard material; the interior of the combined sample slot is made of a porous soft material; a reciprocating motion unit along the horizontal direction and a strain loading unit along the vertical direction are shared by the plurality of working stations; the two units cooperatively work for applying mechanical stimulations in different forms, such as, dynamic compression strain and sliding friction, to the sample. The in vitro dynamic culture system disclosed by the invention has a simple structure, convenience in operation and small size and can be used in a sterile operation platform; a porous soft chamber in the combined sample slot can supply a bionic flexible clamping environment and a dispersing feeding nutrition supply condition to a tissue sample; an effective method is supplied for in vitro dynamic culture and functional assessment of active tissues, such as, cartilago articularis.