The invention discloses a gravity-driven integratable relief type microfluidic chip and application thereof, and belongs to the technical field of microfluidic chips. The gravity-driven integratable relief type microfluidic chip is formed by splicing a plurality of chips via splicing ports, wherein the surface of each chip is of a relief structure; the relief type microfluidic chip is designed with eight functions, including conveying, screening, splitting, integration, oscillation, jumping, replacing and collecting. The relief type microfluidic chip has the advantages that a microfluidic channel structure with certain special movement track control function and a splicing port structure at an outer edge are contained; the chips are flexibly spliced and integrated according to set purpose, and the gravity driving and the super-hydrophobic modifying of the chip surface are combined, so that the liquid droplet can subject to high-speed, multi-channel and functional movement track control.