The invention provides bionic self-healing asphalt and a preparation method thereof. The bionic self-healing asphalt is prepared from matrix asphalt and a bionic self-healing asphalt modifier, wherein the bionic self-healing asphalt modifier is prepared from a self-healing component. A material which has swelling property and autonomously propagates and crystalizes at normal temperature is taken as the self-healing component, and is combined with the common matrix asphalt in the art to simulate a human body healing system, so that on the premise of not affecting the pavement performance of swelling the asphalt, the obtained bionic self-healing asphalt enables a road to have an autonomous healing function like a human body; the bionic self-healing asphalt has the functions of swelling and autonomously propagating and crystalizing, the preparation method is simple, the production cost is low, and the prepared bionic self-healing asphalt has the effects of autonomously healing cracks and effectively reducing cracking, and furthermore other properties of the asphalt are not affected.