The invention discloses an oxidized graphene based light-driven two-layer composite membrane and a preparation method and application thereof. The oxidized graphene based light-driven two-layer composite membrane produces bending deformation under light irradiation and comprises a PDMS and precious metal nano material mixture layer and an oxidized graphene film flatly laid and fixed to the mixture layer and having optical transparency. The preparation method of the oxidized graphene based light-driven two-layer composite membrane comprises the steps that the oxidized graphene film having optical transparency is prepared; the PDMS and precious metal nano material mixture layer is prepared; the two-layer composite membrane is prepared. The oxidized graphene based light-driven two-layer composite membrane produces controllable bending deformation under simulated sunlight irradiation and the coupling effect of optical-thermal-mechanical asymmetric composite structure, has the advantages of being transparent, flexible, large in deformation degree, good in controllability, remotely controllable and operatable and the like and can be applied to a light-driven flexible mechanical device, micro-vale for controlling microfluidics in a micro-fluidic chip and the like.