The invention provides a method for a doctor precisely operating a prostate puncture needle to take out a biopsy, and a calculation method of the spatial location of the puncture needle before a surgery. A traditional sextant biopsy is subjected to an improvement, namely the method that in the past a whole prostate is divided equally into six parts (the top portion of the prostate, the middle portion and the bottom portion of the prostate, the left side and the right side of the prostate) is improved to the method that the puncture is only performed on selected points in an interested area; preoperative image information can be able to provide a clear and stereo prostate lesion area for the doctor, an angle adjusting indicating dial auxiliary device is added, a needle point position, a planning route and a needed puncture rotary angle are calculated based on a preoperative plan so that an improved puncture suit can achieve precise rotary operations, has relatively high accuracy and high individual specificity, and achieves precise puncture.