The invention particularly discloses a comprehensive evaluation method for remediation effects of heavy metal contaminated soil based on plants, soil and microorganisms. The method comprises the steps that (1) a contaminated soil remediation effect evaluation system is constructed; (2) evaluation indicators are determined; (3) the evaluation indicators are subjected to normalization processing; (4) weights of all the evaluation indicators are determined; and (5) an evaluation score of remedied soil is calculated, and comparison is performed in combination with evaluation standards to obtain the remediation effects of the heavy metal contaminated soil treated by different remediation means. According to the evaluation method, the evaluation indicators include three criterion layer factors, nine element layer factors and fourteen indicator layer factors in total and cover content in the aspects of chemistry, microorganisms and plants, farmland functions and contamination remediation actual conditions are comprehensively considered, the method is suitable for comprehensive evaluation of contamination remediation work and large-range remediation work evaluation, and compared with existing evaluation methods, the method is more comprehensive, accurate and scientific.