The invention provides a HoSrMnNi co-doped trigonal bismuth ferrite superlattice film and a preparation method thereof. A Bi<0.89>Ho<0.08>Sr<0.03>Fe<0.94>Mn<0.03>Ni<0.03>O<3>/Bi<0.89>Ho<0.08>Sr<0.03>Fe<0.93>Mn<0.03>Ni<0.04>O<3> superlattice film, i.e., the HoSrMnNi co-doped trigonal bismuth ferrite superlattice film, is prepared from a bismuth ferrite film doped by different elements, wherein the crystal structure of the bismuth ferrite film is a trigonal structure and belongs to both the space group R3c: H and the space group R3c: R. According to the invention, a sol-gel process is employed, and spin-coating and layer-upon-layer annealing methods are utilized; requirements on equipment are simple; and the preparation method is suitable for preparation of films on large surfaces and surfaces with irregular shapes, allows chemical components to be accurate and controllable, and can improve the multiferroic performance of the BiFeO<3> film.