The invention discloses a gene sequencing chip and a gene sequencing method and gene sequencing device corresponding thereto. The gene sequencing chip comprises a display panel including multiple display units, wherein each of the display units includes a transistor and an electrode connected to the first terminal of the transistor; an opening defining layer located on the display panel and including openings corresponding to the display units; and an ion-sensitive membrane having at least a partial region located within the opening and connected to the collector terminal of the transistor.