The invention relates to the technical field of enzymatic activity, in particular to an apple polyphenol oxidase accelerative activator and application thereof. The apple polyphenol oxidase accelerative activator comprises a mixture of dodecyltrimethylammonium bromide or hexadecyl trimethyl ammonium bromide and a Gemini surfactant according to the weight ratio of 10-2,000: 1-4; and the Gemini surfactant is dibromo-bis(dimethyl dodecyl) pentyl diammonium or dibromo-bis(dimethyl hexadecyl) pentyl diammonium. The invention further provides application of the apple polyphenol oxidase accelerative activator in improvement of apple polyphenol oxidase activity. The apple polyphenol oxidase activity can be increased to be 240%, and the use amount level of the apple polyphenol oxidase accelerative activator is low.