The invention discloses a biomarker combination of NPC (Nasopharyngeal Carcinoma), a detection kit and application. According to the biomarker combination, the detection kit and the application, disclosed by the invention, the concentration level of serum markers is obtained by analyzing serums of a patient suffering from the NPC, then bioinformatics analysis is carried out, markers can be selected for carrying out an ELISA (Enzyme-Linked Immuno Sorbent Assay) experiment for further verification, and a result shows that the serum markers of TIMP-2 (Tissue Inhibitor of Metalloproteinase-2), IGF-I (Insulin-like Growth Factor-I), IL-8 (Interleukin-8), SELL (Selectin-L), CCL24, MMP3 (Metal Matrix Proteinase 3), MSP (Macrophage Stimulate Protein)-alpha and HCC-4 (Hepatocellular Carcinoma 4) are closely related to the NPC, can be used for clinical diagnose and prevention inspection and has a very good practical application value. Inspection is directly carried out aiming at the markers in the serums, so that the operation is convenient and fast, and the biomarker combination is suitable for being popularized and applied.