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CN107126559A 一种异育银鲫抗CyHV‑2口服重组芽孢疫苗及其制备方法 失效 - 权利终止

一种异育银鲫抗CyHV‑2口服重组芽孢疫苗及其制备方法
Abstract:
本发明涉及一种异育银鲫抗鲤疱疹病毒II型(CyHV‑2)口服重组枯草芽孢杆菌芽孢疫苗及其制备方法。该疫苗是用异育银鲫颗粒饲料包被表面展示CyHV‑2免疫保护性抗原的重组枯草芽孢杆菌芽孢CyHV‑2而制得；所述芽孢是具有萌发缺陷、无抗生素抗性基因标记的芽孢。其制备方法是构建萌发缺陷、无抗生素抗性基因标记和表面展示CyHV‑2免疫保护性抗原的重组枯草芽孢杆菌芽孢，制备异育银鲫颗粒饲料包被重组芽孢的CyHV‑2口服重组芽孢疫苗，用于CyHV‑2引起的异育银鲫出血病的防治。本发明制备萌发缺陷、无抗生素抗性基因标记的表面展示CyHV‑2保护性抗原的口服重组芽孢疫苗，具有更安全和高效的特点和优势。
Abstract(English):

The invention relates to a Carassius auratus gibelio anti-Cyprinid herpesvirus 2 (CyHV-2) oral recombinant Bacillus subtilis spore vaccine and a preparation method thereof. The vaccine is prepared by coating recombinant Bacillus subtilis spore CyHV-2 having a surface showing a CyHV-2 immune protective antigen with a Carassius auratus gibelio granular feed; and the above spore is a spore with germination defects and no antibiotic-resistant gene markers. The preparation method comprises the following steps: constructing the recombinant Bacillus subtilis spore CyHV-2 having germination defects, having no antibiotic resistance gene markers and having a surface showing the CyHV-2 immune protective antigen, and preparing the Carassius auratus gibelio granular feed coated CyHV-2 oral recombinant Bacillus subtilis spore vaccine used for preventing and treating CyHV-2 induced Carassius auratus gibelio hemorrhage. The oral recombinant Bacillus subtilis spore vaccine having germination defects, having no antibiotic resistance gene markers and having a surface showing the CyHV-2 immune protective antigen, prepared in the invention, has the characteristics of safety and high efficiency.

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