The invention discloses a DBC file analysis method and a DBC file analysis program design method based on CAN communication. The DBC file analysis program design method comprises the following steps: setting CAN communication parameter configuration to realize normal CAN communication connection; creating a DBC file, wherein the DBC file includes a frame information message; writing a DBC file analysis program, and analyzing the number, frame names and frame IDs of given variable assignment frames; dynamically creating the same number of controls as the frames in the frame information message to display frame characters; dynamically creating controls of the signal names in the DBC file to display the names; dynamically creating controls of the signal values in the DBC file to display the signal values; and receiving or sending each frame of data in the frame information message. The methods can satisfy the need of users for monitoring vehicle controller information in real time. Time is saved, the development cost is reduced, and the error rate of file analysis is reduced.