The invention provides a high performance stereocomplex polylactic acid static spinning nanometer fiber film and a preparation method thereof; the method comprises the following steps: 1, using an existing static spinning method to mix levorgyration polylactic acid with dextrorotation polylactic acid according to the mass ratio of 6:4 to 4:6, thus obtaining the polylactic acid static spinning nanometer fiber film, and drying the fiber film under room temperature; 2, annealing the dried polylactic acid static spinning nanometer fiber film for at least 15 minutes at the temperature of 80-100 DEG C; 3, crystallizing the polylactic acid static spinning nanometer fiber film after the annealing treatment for 0.1-4 hours under the interface of 0.5-5MPa in pressure and at the temperature of 170-210 DEG C. The method can greatly improve the polylactic acid static spinning nanometer fiber film mechanical property, hydrolysis resistance property and solvent resistance property, so the fiber film can keep structural integrity in isolation applications, and can be repeatedly used.