The invention discloses a peritoneo-puncture needle fixing device which comprises an air bag and a fixing sleeve, wherein an opening is formed in the middle of the air bag; when the air bag is inflated, the opening is reduced to fix a puncture needle on the air bag; an air groove is formed in the fixing sleeve; the air bag is arranged at one side, back to the air groove, of the fixing sleeve; and after the fixing sleeve is put on the human skin, the air groove is sealed by the human skin, a closed air cavity is formed between the fixing sleeve and the human skin, a second air vent is formed in the fixing sleeve, and a first air vent is formed in the air bag. The fixing sleeve is put on the human skin and the fixing sleeve and the human skin form an air cavity during an operation, air is exhausted from the second air vent, low pressure is formed in the air cavity, and the fixing sleeve is fixed to the human skin; and the air bag is inflated, the opening is reduced, and the puncture needle is clamped in the opening to be fixed in the operation, so that an operator can independently operate the puncture needle to extract seroperitoneum, and the operation does not need to be carried out by two persons.