The invention discloses a TPO automotive trim material with simulated stitching effect. The TPO automotive trim material comprises a sponge layer, a TPO surface layer and a paint from the lower part to the upper part in sequence, wherein the TPO surface layer is prepared by performing extrusion molding on a TPO material; the TPO material comprises the following components in parts by weight: 20 to 40 parts of polypropylene, 10 to 20 parts of low density polyethylene, 5 to 12 parts of polyurethane, 40 to 55 parts of rubber, 12 to 20 parts of tackifying resin, 8 to 14 parts of an inorganic filler, 0 to 8 parts of color master, and 2 to 5 parts of an assistant. The invention further discloses a preparation method of the TPO automotive trim material with simulated stitching effect. The TPO trim material can achieve authentic stitching effect of an automotive trim instrument board or a door sheet, and ensures that automotive trim is high-end oriented; the production technology is simple, the manufacture time can be greatly shortened through a one-step shaping technology, the rejection rate is reduced, and the cost is reduced.