The invention discloses an enzymatic performance improved methyl parathion hydrolase mutant and application thereof. Wild-type enzymatic performance improved methyl parathion hydrolase is targetedly modified, mutation in vitro and expression to OPHC2 are performed by combining with a protein structure calculation and stimulation technology and a molecular biology technology, and kinetic parameters and the stability of protease are detected by applying biochemical means, so that a mutant V117HQ119E with remarkably improved thermal stability and substrate affinity and a mutant F263W with remarkably improved catalytic efficiency and improved stability in a surfactant are obtained through screening, wherein amino acid sequences of the mutant V117HQ119E and the mutant F263W are shown as SEQ ID No. 2 and SEQ ID No. 4 respectively. The enzymatic performance improved methyl parathion hydrolase mutant remarkably improves the enzymatic performance of the OPHC2, widens the application field and the action efficiency of the OPHC2 and has a wide application prospect on the aspects of degradation of an organophosphorus pesticide, organism detoxication, washing and disinfection of a nerve agent and the like.