The invention discloses a type II crystal substance of the compound 990207-22-1 (having a chemical name of 1-piperazinedithiocarbamoto-3-cyano-3,3-diphenylpropyl ester hydrochloride), a preparation method thereof, and a pharmaceutical composition and application thereof. In particular, the invention discloses a type II crystal solid substance form of the compound 990207-22-1 in solid state, a preparation method of the type II crystal sample, and application of the crystal substance of 990207-22-1 as an active ingredient in the preparation of antitumor and tumor-preventive drugs.