The invention discloses an intelligent sterile hand-washing and glove-wearing machine. Through mechanized sterile equipment, hand washing and glove wearing are automatically conducted, not only are the time and labor saved, but also glove infection is avoided, and the risks of operations are reduced. In order to solve the technical problems, according to the technical scheme, a hand washing system and a glove wearing system are both arranged on a cabinet, the hand washing system is located at one end of the cabinet, and the glove wearing system is located at the other end of the cabinet; according to the structure of the hand washing system, a hand washing machine is arranged on the upper portion of the cabinet, a spray-wash mechanism is arranged in the hand washing machine, an enclosed mechanism is arranged at an opening of the hand washing machine and is matched with the hand washing machine to form an enclosed spray-wash cavity; the intelligent sterile hand-washing and glove-wearing machine further includes a water storage tank, a spray head, a brush arm, a dryer, an exhaust fan, a light sensor and a controller, through the light sensor which senses the hands and the controller which controls the operation of a water tank, the spray head, the brush arm, the dryer and the exhaust fan, and the purpose is achieved that the hands are cleaned. The intelligent sterile hand-washing and glove-wearing machine can be widely applied in the field of medical treatment, chemical industry and food.