The invention provides a novel yeast inducible promoter and application thereof and belongs to the technical field of molecular biology. According to the technical scheme, the DNA (deoxyribonucleic acid) sequence of the novel yeast inducible promoter is shown as a sequence 1 in a sequence table, and the length of the novel yeast inducible promoter is 673 bp. The novel yeast inducible promoter has the advantage that the novel yeast inducible promoter DDI2 can be effectively induced by cyanamide; the strength of the novel yeast inducible promoter DDI2 is close to that of a known inducible promoter GAL1 and higher than that of a constitutive promoter ADH1; during induction, the novel yeast inducible promoter can save change of mediums and control the expression quantity of genes through inducing time and amount of inducing agent, thereby having advantages over the common GAL1 in terms of time cost and price cost. Detecting results of a flow cytometer shows that the strength of the promoter DDI2 is higher than that of the promoter ADH1 and close to that of the promoter GAL1, the required inducing time of the promoter DDI2 is shorter than that of the promoter GAL1, so that change of the mediums can be saved; according to the detecting results of Western and the flow cytometer, the strength of the promoter DDI2 can reach a maximum when the concentration of the inducing agent is 5-8 mM.