The invention discloses a method for preparing a lutein crystal oil-suspended injection and belongs to the technical field of health product processing. The method comprises the following steps: (1) preparing lutein crystal; (2) adding an emulsifier according to the mass ratio of vegetable oil to the emulsifier of (50:1) to (100:7) and stirring to obtain a mixed liquid; (3) adding an antioxidant according to the mass ratio of the mixed liquid to the antioxidant of (250:1) to (200:1), further stirring, adding lutein crystal powder and carrying out phased shearing emulsification; and (4) after emulsification is completed, cooling to room temperature to obtain the lutein oil-suspended injection. The invention provides the preparation method for the lutein crystal oil-suspended injection which is high in stability and long in storage time.