The invention belongs to the field of environmental sample detection, and particularly relates to a detection method of 2-phenylbenzimidazole-5-sulfonic acid (PBSA) in soil. At present, studies on PBSA mainly focus on content levels in cosmetics and products, and a degradation mechanism in a water environment; but, almost no study focuses on a detection method and a content level of PBSA in the soil; therefore, in order to learn a concentration level and an environmental behavior of PBSA in a soil environment, an efficient, quick and highly sensitive analysis method is required to be established for qualitative and quantitative detection of a trace amount of 2-phenylbenzimidazole-5-sulfonic acid in the soil; and the invention aims at providing an analysis and detection method of PBSA in the soil, in particular to an analysis method using ultrasonic extraction, silica gel chromatographic column purification and high-performance liquid chromatograph-mass spectrometer detection.