The invention provides a screen detection box for stabilizing and fixing objects. The screen detection box comprises a box body, a box cover and a plurality of first object positioning assisting mechanisms, wherein a plurality of first storage slots are formed on the box body; the box cover is rotatably arranged on the box body; the first object positioning assisting mechanisms include a plurality of clamping plates, a plurality of first resetting component sets and a plurality of guiding shaft sets; a plurality of mounting slot sets are arranged on the box body; the mounting slot sets are one-to-one corresponding to the first storage slots; the mounting slot sets include a plurality of mounting slots; the mounting slots are communicated with the first storage slots; the clamping plates are one-to-one corresponding to the first storage slots; the first resetting component sets are one-to-one corresponding to the first storage slots; the first resetting component sets include a plurality of first resetting components; the first resetting components are connected with the inner walls of the mounting slots and the clamping plates; the guiding shaft sets are one-to-one corresponding to the clamping plates; the guiding shaft sets include a plurality of guiding shafts; the guiding shafts are arranged on the clamping plates in the rotatable form. The screen detection box is conveniently used and is easy for quickly and stably clamping the bar-shaped objects.