The invention belongs to the technical field of plant tissue culture, and particularly relates to a blueberry tissue culture seedling detoxification method and culture method. The detoxification method comprises the following steps: (1) heat treatment: treating a blueberry tissue culture seedling at 35-50 DEG C for 3-3.5 days; and (2) chemical agent treatment: cutting off a stem apex of the tissue culture seedling treated by the step (1), putting the stem apex in a virazole-containing culture medium, and continuing culture at 21-25 DEG C for 45-55 days, wherein the concentration of the virazole is 0.2-0.3 mg/L. On such basis, the invention also provides a blueberry tissue culture seedling culture method containing the detoxification method. According to the method, the two-stage detoxification treatment is performed to greatly enhance the detoxification rate of the blueberry tissue culture seedling, and the survival rate of the tissue culture seedling is basically not influenced. The detoxified blueberry seedling can not mutate after being transplanted to the field. Therefore, the methods provided by the invention have broad market prospects and important application and popularization values.