The invention provides collagen secretion promotion ultrashort peptides and application thereof, and belongs to the technical field of biomedicine. The two kinds of collagen secretion promotion ultrashort peptides Magin-FL and Purin-WH3 have the capability of promoting the collagen synthesis; in addition, the characteristics of ultra-high oxidization resistance, aging-resistant activity, no cell toxicity, no hemolysis activity, simple preparation method and the like are realized, so that the collagen secretion promotion ultrashort peptides are used for preparing medicine for treating injured skin restoration and regeneration such as medicine for treating burnt injury, scald injury and skin ulceration, and can replace EGF to be used in the fields of anti-aging and anti-oxidization beatification skin care product new raw materials for skin restoration and skin collagen proliferation simulation and the like. In addition, the effects are possibly not limited to the field. The ultrashort peptides Magin-FL and Purin-WH3 have small molecular weight and simple structure; the used chemical synthesis method has very low cost; the ultrashort peptides Magin-FL and Purin-WH3 have the obvious beneficial characteristics through being compared with EFG with the sale price being nearly 2 million U.S dollars per gram.