The invention discloses an anti-microbial and anti-oxidation nano film and a preparation method for the same. The preparation method comprises the steps that a cyclodextrine aqueous solution is mixed with orange essential oil, and an orange essential oil/cyclodextrine clathrate compound is obtained after crystallization; and the orange essential oil/cyclodextrine clathrate compound, a spinning dope and glucose are mixed, a spinning solution is obtained, and the anti-microbial and anti-oxidation nano film is obtained by a static spinning technology. The prepared anti-microbial and anti-oxidation nano film has outstanding anti-microbial and anti-oxidation effects; the film can be applied to food packaging; and the film can effectively restrain growth of microorganism on food surfaces, prevent oxidative deterioration of foods, prolong shelf life of foods and guarantee food quality.