The invention provides a cattle brucellosis fluorescent-labeled immunochromatography test strip, and belongs to the technical field of protein engineering. The cattle brucellosis fluorescent-labeled immunochromatography test strip is characterized in that the mixed antigen of cattle brucellosis outer membrane proteins OMP19, OMP22 and OMP28, goat anti-rabbit IgG, and rabbit anti-goat IgG are covered to an NC (nitrocellulose) membrane, and fluorescent-labeled rabbit anti-ox IgG and goat anti-ox IgG are covered to a glass fiber membrane, so as to detect the brucellosis antibody in the specimen. The cattle brucellosis fluorescent-labeled immunochromatography test strip has the advantages that the operation is simple, convenient and quick, the special instrument equipment is not needed, the professional training is not needed, the result is clear and distinguishable, and the test strip is suitable for being popularized, and is suitable for basic levels; the test strip is suitable for field detection and epidemics investigation, the assisting function on the diagnosis of brucellosis infection is realized, and the good market application prospect is realized.