The invention provides a pixel driving circuit, a driving method thereof, a display panel and a driving method thereof. The pixel driving circuit comprises a left-eye pixel driving sub-circuit and a right-eye pixel driving sub-circuit. The left-eye pixel driving sub-circuit comprises a left-eye driving transistor, a left-eye storage capacitor unit, a left-eye data writing unit and a left-eye light-emitting control unit. The right-eye pixel driving sub-circuit comprises a right-eye driving transistor, a right-eye storage capacitor unit, a right-eye data writing unit and a right-eye light-emitting control unit. The pixel driving circuit comprises the left-eye pixel driving sub-circuit and the right-eye pixel driving sub-circuit which operate alternately. Therefore in the displaying time of each picture frame, the displaying time length of the left-eye picture and the displaying time length of the right-eye picture are about one half of the displaying time length of the picture frame, thereby preventing flashing in displaying and improving displaying of the displayed picture.