The invention discloses a method for rapid tissue propagation of acer saccharum, and belongs to the technical field of woody plant tissue culture and propagation. The method comprises the following steps: selecting explants, pretreating the explants, establishing a sterile system, performing inductive culture, performing multiplication culture, performing rooting culture, strengthening and domesticating seedlings, and the like. Tissue organs of plants are cultured under a sterile system condition by controlling external environment conditions artificially, the purpose of rapidly propagating nursery stock is achieved, and stable properties of excellent female parents are effectively maintained. Compared with common vegetative propagation of cuttage and grafting, the method is small in material amount, free of limit of natural conditions and relatively applicable to neophytes, the propagation coefficient is as high as 5-20 times, and propagation and popularization problems caused by shortage of resources of the acer saccharum are solved.