The invention discloses a method for inducing callus and rapidly reproducing didiplis diandra. The method comprises steps as follows: A, raw material selection and pretreatment: a plant stem which has a complete growing point and is bright green in color and robust is selected to serve as an explant and pretreated with a detergent and distilled water; B, preparation of a sterile explant: the explant is treated with alcohol and mercuric chloride, and the sterile explant is prepared; C, callus and cluster bud induction: a proper hormone variety and concentration combination condition is determined, the explant is put into an induction culture medium, and callus and cluster buds are induced; D, callus differentiation and proliferation culture: the hormone combination is adjusted, the callus is put into a proliferation culture medium for proliferation culture, and the cluster buds are generated through differentiation; E, rooting culture: a hormone variety and concentration combination are selected, and rooting culture is performed; F, seedling hardening and transplanting. The method can be not limited by seasons and environments, sterile plants can be cultured, and ornamental plants can be provided for the aquarium industry constantly.