The invention discloses a laparoscopic varicocele ligation puncture device. Such structure design that a fixing mechanism and an anti-disengagement mechanism is adopted, the expansion stretch-out and draw-back principle of an elastic air bag and the adsorption principle of a carbon nano tube array are made use of, a conical head can be effectively prevented from being disengaged from a puncture part, a main sheath tube thus does not move in the human body or outside the human body in the operation process, the operability is largely improved, meanwhile an air guide hole is formed in the conical head, the air inflation effect of a veress needle can be achieved, and the safe use function requirements of puncture and pneumoperitoneum are met.